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Key Documents

MAK551

Sigma-Aldrich

Butyrylcholinesterase (BChE) Activity Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
41116104
NACRES:
NA.21

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; obesity; cardiovascular diseases

shipped in

dry ice

storage temp.

−20°C

General description

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized in liver and present in blood. BChE is a nonspecific cholinesterase enzyme and can hydrolyze many different choline esters, serving as the first line of defense against toxic compounds reaching the bloodstream. ​

The Butyrylcholinesterase Activity Assay Kit is based on a synthetic butyrylthiocholine based substrate, which can be hydrolyzed by BChE and produce thiocholine. Thiocholine can react with 5, 5′-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be detected at 410 nm. The assay is convenient, sensitive and can detect as low as 6 mU/mL in a variety of samples.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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