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CS0790

Sigma-Aldrich

Antioxidant Assay Kit

sufficient for 200 tests

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NACRES:
NA.84

usage

sufficient for 200 tests

Quality Level

detection method

colorimetric

shipped in

wet ice

storage temp.

2-8°C

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This Item
MAK334MAK094354102
vibrant-m

CS0790

Antioxidant Assay Kit

vibrant-m

MAK334

Antioxidant Assay Kit

vibrant-m

MAK094

Protein Carbonyl Content Assay Kit

vibrant-m

354102

Glutathione Assay Kit

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

usage

sufficient for 200 tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 tests

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.

Application

Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity.
It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.

Suitability

Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates

Principle

The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS·+ , a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.

Preparation Note

Reagents are to be prepared with ultrapure water.

Reconstitution

Myoglobin Solutions are to be stored at -20C after reconstitution.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.

Legal Information

Kit Components Only

Product No.
Description

  • Assay Buffer 10X 30 mL

  • Stop Solution 20 mL

  • Hydrogen Peroxide, 3% solution 1 mL

  • Myoglobin from horse heart 2 x 1

  • (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1

  • ABTS, 10 mg tablets 4 tab

  • Phosphate-Citrate Buffer, pH 5 4 tab

related product

Product No.
Description
Pricing

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Reversal of hepatic steatosis by omega?3 fatty acids measured non?invasively by 1H?magnetic resonance spectroscopy in a rat model.
Marsman HA, et al.
Journal of Gastroenterology and Hepatology, 26(2), 356-363 (2011)
Dave C Francisco et al.
Free radical biology & medicine, 44(4), 558-569 (2007-11-17)
Oxidatively induced stress and DNA damage have been associated with various human pathophysiological conditions, including cancer and aging. Complex DNA damage such as double-strand breaks (DSBs) and non-DSB bistranded oxidatively induced clustered DNA lesions (OCDL) (two or more DNA lesions
Use of sourdough fermentation and pseudo-cereals and leguminous flours for the making of a functional bread enriched of γ-aminobutyric acid (GABA).
Coda R, et al.
International Journal of Food Microbiology, 137(2), 236-245 (2010)
Grit Kasper et al.
Stem cells (Dayton, Ohio), 27(6), 1288-1297 (2009-06-06)
Progenitor cells such as mesenchymal stem cells (MSCs) have elicited great hopes for therapeutic augmentation of physiological regeneration processes, e.g., for bone fracture healing. However, regeneration potential decreases with age, which raises questions about the efficiency of autologous approaches in
Muhammad Arshad et al.
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Alfalfa (Medicago sativa) is an important forage crop that is often grown in areas that frequently experience drought and water shortage. MicroRNA156 (miR156) is an emerging tool for improving various traits in plants. We tested the role of miR156d in

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