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615700

Sigma-Aldrich

Total Antioxidant Status Assay Kit

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usage

sufficient for 50 tests

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

assay range

standard curve range: 0-2.5 mM
(plasma)

input

sample type serum
sample type plasma
sample type tissue extract(s)
sample type beverage(s)
sample type plant extract(s)

detection method

colorimetric

shipped in

ambient

storage temp.

2-8°C

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Total Antioxidant Status Assay Kit

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manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

-

storage condition

OK to freeze

storage condition

-

assay range

standard curve range: 0-2.5 mM
(plasma)

assay range

-

assay range

-

assay range

-

input

sample type serum
sample type plasma
sample type tissue extract(s)
sample type beverage(s)
sample type plant extract(s)

input

-

input

sample type cell lysate
sample type tissue homogenate(s)
sample type erythrocyte lysate
sample type plasma
sample type serum

input

-

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

General description

A colorimetric assay kit for the measurement of the total antioxidant status. Each kit is suitable for performing a minimum of 50 assays.
Note: Spectrophotometer equipped with a temperature-controlled cuvette holder is strongly recommended for use with this kit.

Components

Substrate, Chromogen, Standard, Buffer, and a user protocol.

Warning

Toxicity: Standard Handling (A)

Principle

The Calbiochem Total Antioxidant Status Assay Kit is designed to assay antioxidant levels in serum or plasma samples. Additionally, the assay may be used to measure the antioxidant potential of (suitably solubilized) food and drug samples.

Preparation Note

• Chromogen: Add 10 ml Buffer to each vial of Chromogen needed for the assay (10 tests per vial). After reconstitution, the Chromogen is stable for 2 days at 4°C or 8 h at room temperature.

• Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.

• Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.

Storage and Stability

Upon arrival store the entire kit contents at 4°C. Note: The concentration of the standard is lot-specific. Please refer to the vial label for lot-specific concentration.

Analysis Note

Calculation of Total Antioxidant StatusThe antioxidant concentration of the Standard is used to calculate the antioxidant levels in the samples.

1. Determine the ΔA for the samples, standard, and blank. ΔA = A - Ao
2. Calculate the concentration of antioxidants in the sample using the formula below:
<div class="Bio_doc_image">Figure 2: Antioxidant Concentration Formula
</div>
NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.

Other Notes

Miller, N.J., et al. 1993. Clin. Science 84, 407.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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