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CAT100

Sigma-Aldrich

Catalase Assay Kit

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

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NACRES:
NA.84

Quality Level

usage

sufficient for ≥100 tests enzymatic

detection method

colorimetric

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... CAT(847)

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CAT100

Catalase Assay Kit

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CS0260

Glutathione Assay Kit

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CS0170

Thioredoxin Reductase Assay Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

usage

sufficient for ≥100 tests enzymatic

usage

sufficient for 100 tests

usage

sufficient for 700 assays

usage

 kit sufficient for 100 assays (1 mL)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

detection method

colorimetric

detection method

colorimetric

detection method

-

detection method

-

General description

The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.

Application

Sutitable for Colorimetric and UV Assays

Biochem/physiol Actions

Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.

Features and Benefits

  • Useful for determining catalase activity - may be used in various tissues and cells
  • Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity

Suitability

Suitable for studying catalase activity in various tissues and subcellular organelles.

Principle

This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm

Unit Definition

One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.

Preparation Note

Use ultrapure water in preparation of all solutions.

Kit Components Also Available Separately

Product No.
Description
SDS

  • P6782Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol), 950-2000 units/mg solid (using ABTS) 5 mgSDS

  • 323381Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2OSDS

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Customers Also Viewed

Slide 1 of 5

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M Zámocký et al.
Progress in biophysics and molecular biology, 72(1), 19-66 (1999-08-14)
This review gives an overview about the structural organisation of different evolutionary lines of all enzymes capable of efficient dismutation of hydrogen peroxide. Major potential applications in biotechnology and clinical medicine justify further investigations. According to structural and functional similarities
M B Hampton et al.
FEBS letters, 414(3), 552-556 (1997-10-10)
The induction of apoptosis in Jurkat T-lymphocytes with 50 microM hydrogen peroxide was associated with caspase activation. Caspase activity was first detected 3 h after treatment, and the morphological features of apoptosis were apparent by 6 h. At higher concentrations
A J Kowaltowski et al.
FEBS letters, 473(2), 177-182 (2000-05-17)
The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae
Susmita Das Nishu et al.
PloS one, 14(3), e0213370-e0213370 (2019-03-13)
Algicidal bacteria have received broad acceptance as an ecofriendly tool for controlling harmful algal blooms. However, their practical application is still limited to the lab-scale tests due to the complex alga-bacterium interactions in different nutrient statuses. In this study, the
S Tada-Oikawa et al.
FEBS letters, 442(1), 65-69 (1999-01-29)
Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant

Articles

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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