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C9322

Sigma-Aldrich

Catalase from bovine liver

lyophilized powder, 2,000-5,000 units/mg protein

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Synonym(s):
H2O2:H2O2 oxidoreductase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

biological source

bovine liver

Quality Level

form

lyophilized powder

specific activity

2,000-5,000 units/mg protein

mol wt

tetramer ~250 kDa

isoelectric point

5.4

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

Gene Information

cow ... CAT(280743)

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This Item
C40E3289C30
Catalase from bovine liver lyophilized powder, 2,000-5,000 units/mg protein

C9322

Catalase from bovine liver

Catalase from bovine liver lyophilized powder, ≥10,000 units/mg protein

C40

Catalase from bovine liver

Catalase from bovine liver ≥20000 units/mg protein, lyophilized powder

E3289

Catalase from bovine liver

Sigma-Aldrich

C30

Catalase from bovine liver

Gene Information

cow ... CAT(280743)

Gene Information

cow ... CAT(280743)

Gene Information

-

Gene Information

cow ... CAT(280743)

specific activity

2,000-5,000 units/mg protein

specific activity

≥10,000 units/mg protein

specific activity

≥20000 units/mg protein

specific activity

10,000-40,000 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

aqueous suspension

mol wt

tetramer ~250 kDa

mol wt

tetramer ~250 kDa

mol wt

tetramer ~250 kDa

mol wt

tetramer ~250 kDa

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.
Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Preparation Note

This product is a lyophilized powder with activity of 2,000-5,000 units/mg.
The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

inhibitor

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Christopher Gell et al.
Methods in cell biology, 95, 221-245 (2010-05-15)
In vitro assays that reconstitute the dynamic behavior of microtubules provide insight into the roles of microtubule-associated proteins (MAPs) in regulating the growth, shrinkage, and catastrophe of microtubules. The use of total internal reflection fluorescence microscopy with fluorescently labeled tubulin
James C Dooley et al.
Current biology : CB, 30(12), 2404-2410 (2020-05-16)
Cortical development is an activity-dependent process [1-3]. Regarding the role of activity in the developing somatosensory cortex, one persistent debate concerns the importance of sensory feedback from self-generated movements. Specifically, recent studies claim that cortical activity is generated intrinsically, independent
Julie Elizabeth Keeble et al.
Pain, 141(1-2), 135-142 (2008-12-09)
Inflammatory diseases associated with pain are often difficult to treat in the clinic due to insufficient understanding of the nociceptive pathways involved. Recently, there has been considerable interest in the role of reactive oxygen species (ROS) in inflammatory disease, but
Eduard N Lavrentyev et al.
American journal of physiology. Heart and circulatory physiology, 296(1), H106-H118 (2008-11-04)
In rat diabetic animal models, ANG(1-7) treatment prevents the development of cardiovascular complications. Angiotensin-converting enzyme (ACE)2 is a major ANG(1-7)-generating enzyme in vascular smooth muscle cells (VSMCs), and its expression is decreased by a prolonged exposure to high glucose (HG)
Manuel A Pallero et al.
Journal of vascular research, 47(4), 309-322 (2009-12-18)
Despite advances in stent design, in-stent restenosis (ISR) remains a significant clinical problem. All implant metals exhibit corrosion, which results in release of metal ions. Stainless steel (SS), a metal alloy widely used in stents, releases ions to the vessel

Articles

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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