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MAK528

Sigma-Aldrich

Superoxide Dismutase (SOD) Assay Kit

new

sufficient for 100 colorimetric tests

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Quality Level

application(s)

cosmetics
pharmaceutical

detection method

colorimetric

relevant disease(s)

amyotrophic lateral sclerosis (ALS); neurological disorders; musculoskeletal disorders; cancer; neurodegenerative disorders; pulmonary disorders; cardiovascular diseases

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Superoxide dismutases are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer.

Features and Benefits

  • Linear detection range: 0.05 - 3 U/mL
  • Compatible with high-throughput handling systems.
  • No wash and reagent transfer steps are required.

Suitability

The kit is suitable for the determination of superoxide dismutase in blood, cell, tissue and other biological samples.

Principle

The Superoxide Dismutase (SOD) Assay Kit provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a WST-1 dye to form a colored product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. The color intensity at 440 nm is used to determine the SOD activity in a sample.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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