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X4376

Sigma-Aldrich

Xanthine Oxidase from bovine milk

lyophilized powder, 0.4-1.0 units/mg protein

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Synonym(s):
XOD, Xanthine:oxygen oxidoreductase
CAS Number:
Enzyme Commission number:
1.17.3.2.
EC Number:
MDL number:
NACRES:
NA.54

biological source

bovine milk

Quality Level

form

lyophilized powder

specific activity

0.4-1.0 units/mg protein

mol wt

283 kDa

composition

Protein, 10.0-25.0%

storage temp.

2-8°C

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This Item
X4875X4500X1875
Xanthine Oxidase from bovine milk lyophilized powder, 0.4-1.0 units/mg protein

X4376

Xanthine Oxidase from bovine milk

Sigma-Aldrich

X4875

Xanthine Oxidase from bovine milk

Xanthine Oxidase from bovine milk Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

X4500

Xanthine Oxidase from bovine milk

Sigma-Aldrich

X1875

Xanthine Oxidase from bovine milk

biological source

bovine milk

biological source

bovine milk

biological source

bovine milk

biological source

-

form

lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

283 kDa

mol wt

-

mol wt

-

mol wt

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

General description

Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used as a source for superoxide generation. It has also been used as a model enzyme in the internally calibrated electrochemical continuous enzyme assay (ICECEA).

Biochem/physiol Actions

The isoflavanoid genistein was shown to inhibit xanthine oxidase at concentrations between 0.1-4.0 μM and then activate xanthine oxidase at concentrations higher than 5 μM.
Xanthine Oxidase acts as a potential biomarker for oxidative stress. It is also implicated in tissue and vascular injuries such as liver damage, inflammatory diseases and chronic heart failure.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Contains 0.5% sodium salicylate.

Analysis Note

Protein determined by biuret

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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