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10110434001

Roche

Xanthine Oxidase (XOD)

from cow milk

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Synonym(s):
XOD
Enzyme Commission number:

biological source

bovine milk

Quality Level

form

suspension

specific activity

~1 units/mg protein (At 25 °C with xanthine as the substrate.)

packaging

pkg of 1 mL (20 U)

manufacturer/tradename

Roche

optimum pH

8.5-9.0

shipped in

wet ice

storage temp.

2-8°C

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X1875X4500X4875
Xanthine Oxidase (XOD) from cow milk

10110434001

Xanthine Oxidase (XOD)

Sigma-Aldrich

X1875

Xanthine Oxidase from bovine milk

Xanthine Oxidase from bovine milk Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

X4500

Xanthine Oxidase from bovine milk

Sigma-Aldrich

X4875

Xanthine Oxidase from bovine milk

specific activity

~1 units/mg protein (At 25 °C with xanthine as the substrate.)

specific activity

≥0.4 units/mg protein

specific activity

≥0.8 units/mg protein

specific activity

≥0.1 units/mg protein

form

suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

optimum pH

8.5-9.0

optimum pH

-

optimum pH

-

optimum pH

-

General description

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.
Xanthine:oxygen oxidoreductase

Application

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.
Xanthine Oxidase has been used to study tyrosine nitration.

Biochem/physiol Actions

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Quality

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Sequence

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Unit Definition

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E293nm = 12.2 mmol -1 x L x cm-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Physical form

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Preparation Note

Activator: O2
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Peroxynitrite (ONOO(-)) is a potent nitrating and oxidizing agent that is formed by a rapid reaction of nitric oxide (NO) with superoxide anion (O(2)). It appears to be involved in the pathophysiology of many inflammatory and neurodegenerative diseases. It has
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