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06-735

Sigma-Aldrich

Anti-Caspase 3 Antibody

Upstate®, from rabbit

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Synonym(s):
Anti-CASP-3, Anti-Caspase-3
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)

General description

Caspase-3 (UniProt: P42574; also known as EC:3.4.22.56, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1) is encoded by the CASP3 (also known as CPP32) gene (Gene ID: 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is a heterotetrameric enzyme that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Caspase-3 is initially produced with a propeptide sequence (aa 1-9), the removal of which yields the 268 aa. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (aa 10-175) fragment and the p12 (aa 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (aa 29-175) subunit. The p17 and p12 subunits dimerize and forms the active caspase-3 enzyme. Caspase-3 has a strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. Caspase-3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a Asp216-|-Gly217 bond. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10.

Specificity

Recognizes full-length Caspase 3 (Yama/Apopain) and proteolytic fragments.

Immunogen

Human full-length Caspase 3 fusion protein containing a histidine-6 tag

Application

This Anti-Caspase 3 Antibody is validated for use in Immunihistochmistry and Western Blotting for the detection of Caspase 3.
Western Blotting Analysis: 1μg/mL of this antibody detects Caspase-3 in A431 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Caspase-3 in Human tonsil tissue sections.

Quality

routinely evaluated by immunoblot on RIPA lysates from non-stimulated human A431 cells, mouse 3T3/A31 or rat PC12 cells

Target description

32 kDa

Linkage

Replaces: 04-1090; 04-439

Physical form

Format: Purified
Protein A purified IgG in of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl,and 0.05% sodium azide.

Storage and Stability

Stable for 2 years at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 1


Certificates of Analysis (COA)

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M Leist et al.
Biochemical and biophysical research communications, 258(1), 215-221 (1999-05-01)
The endogenous mediator nitric oxide (NO) blocked apoptosis of Jurkat cells elicited by staurosporine, anti-CD95 or chemotherapeutics, and switched death to necrosis. The switch in the mode of cell death was dependent on the ATP loss elicited by NO. This
Antiandrogen-induced cell death in LNCaP human prostate cancer cells.
Lee, EC; Zhan, P; Schallhom, R; Packman, K; Tenniswood, M
Cell Death and Differentiation null
A caspase cascade regulating developmental axon degeneration.
Simon, DJ; Weimer, RM; McLaughlin, T; Kallop, D; Stanger, K; Yang, J; O'Leary et al.
The Journal of Neuroscience null
Prisca Boisguérin et al.
Cardiovascular research, 116(3), 633-644 (2019-05-31)
Regulated cell death is a main contributor of myocardial ischaemia-reperfusion (IR) injury during acute myocardial infarction. In this context, targeting apoptosis could be a potent therapeutical strategy. In a previous study, we showed that DAXX (death-associated protein) was essential for
Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue.
Chen, CC; Lin, WC; Kong, MS; Shi, HN; Walker, WA; Lin, CY; Huang, CT; Lin, YC; Jung, SM; Lin, TY
The British Journal of Nutrition null

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