MilliporeSigma
All Photos(2)

Documents

C8487

Sigma-Aldrich

Anti-Caspase 3, Active antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Activated Caspase 3 Antibody, Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit, Anti-Apopain, Anti-CPP32, Anti-Yama
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, bovine, human, pig, canine, mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine
microarray: suitable
western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C9598C7729AB3623
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

UniProt accession no.

P42574

UniProt accession no.

P42574

UniProt accession no.

P55211

UniProt accession no.

P42574

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.
Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.

Immunogen

Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.

Application

Anti-Caspase 3, Active antibody has been used:
  • for western blotting of cytochrome c for caspase activation
  • as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
  • in Western blot analysis of activated caspase 3
  • as a primary antibody in immunodetection of rat brain sections

Biochem/physiol Actions

Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma
Liu PF, et al.
Testing, 12(7) (2017)
Caspases: an apoptosis mediator
Palai T K and Mishra S R
Journal of Advanced Veterinary and Animal Research, 2(1) (2015)
Identification of SMURF1 as a possible target for 7q21. 3?22.1 amplification detected in a pancreatic cancer cell line by in?house array?based comparative genomic hybridization.
Suzuki A, et al.
Cancer Science, 99(5), 986-994 (2008)
Kalina Andreeva et al.
Ophthalmology and eye diseases, 6, 43-54 (2014-09-12)
Ischemia/reperfusion (IR) injury has been associated with several retinal pathologies, and a few genes/gene products have been linked to IR injury. However, the big picture of temporal changes, regarding the affected gene networks, pathways, and processes remains to be determined.
Ludger Leifeld et al.
The American journal of pathology, 168(3), 778-785 (2006-03-02)
Apoptosis is a key mechanism underlying fulminant hepatic failure. The role of gelsolin in such apoptotic pathways is not well understood because both pro-apoptotic and anti-apoptotic effects have been reported in vitro, depending on the cell type and in vitro

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service