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CASP3C

Sigma-Aldrich

Caspase 3 Assay Kit, Colorimetric

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NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
sufficient for 100 standard tests (1 ml)

Quality Level

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

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CASP3FAPT131APT165
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CASP3C

Caspase 3 Assay Kit, Colorimetric

vibrant-m

CASP3F

Caspase 3 Assay Kit, Fluorimetric

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Other Notes

Use 17 megohm water only

Kit Components Only

Product No.
Description

  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS

  • A2559Ac-DEVD-pNA substrate 15 mgSDS

  • p-Nitroaniline standard 1 mg

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

415.4 °F - closed cup

flash_point_c

213 °C - closed cup


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