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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

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EC Number:
NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

Quality Level

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

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CASP3CAPT423235419
vibrant-m

CASP3F

Caspase 3 Assay Kit, Fluorimetric

vibrant-m

CASP3C

Caspase 3 Assay Kit, Colorimetric

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

100

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−70°C

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

usage

-

usage

sufficient for 96 tests

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

Product No.
Description

  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS

  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS

  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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