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APT131

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Caspase 3 Colorimetric Activity Assay Kit, DEVD

Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

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Synonym(s):
CPP32
eCl@ss:
32161000

Quality Level

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable

NCBI accession no.

UniProt accession no.

detection method

colorimetric

shipped in

dry ice

Gene Information

human ... CASP3(836)

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CASP3F

Caspase 3 Assay Kit, Fluorimetric

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

200

species reactivity (predicted by homology)

mammals

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

mammals

species reactivity (predicted by homology)

-

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

UniProt accession no.

P42574

UniProt accession no.

-

UniProt accession no.

P42574, Q14790

UniProt accession no.

-

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.

Application

Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Research Category
Apoptosis & Cancer

Components

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution

Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

Storage and Stability

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Tibet Kacira et al.
Neurosurgery, 60(4), 674-679 (2007-04-07)
The purpose of this study is to explore whether or not the levels of caspase-3 (Casp3), neuron-specific enolase (NSE), and high-sensitivity C-reactive protein (hsCRP) were elevated in cerebrospinal fluid (CSF) and serum of patients after aneurysmal subarachnoid hemorrhage (SAH). This
Giuseppe M Campo et al.
Molecular and cellular biochemistry, 311(1-2), 167-177 (2008-01-29)
Previous studies suggest that Transforming growth factor-1beta (TGF-1beta) administration in human fibroblasts exposed to oxidative stress is able to modulate hyaluronan synthases (HASs). HAS modulation in turn increases high molecular weight (Hyaluronan) HA concentration. Nuclear factor kB (NF-kB) is a
Lingfang Yu et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 28(5), 879-890 (2015-07-26)
Lanthanide elements have been documented to possess various biologic effects, and their compounds have been studied intensely for their anti-cancer potential. However, the underlying mechanisms remain largely unknown. In the present study, we propose that the levels of proliferation and
Giuseppe M Campo et al.
Journal of applied toxicology : JAT, 28(4), 509-517 (2007-09-20)
Oxidative stress, inflammation and apoptosis play a critical role in the onset and progression of cellular damage. It was previously reported that hyaluronan (HA) and chondroitin-4-sulphate (C4S) were able to protect human skin fibroblasts from oxidative stress. This antioxidant activity
Anna Zuppini et al.
Journal of cell science, 117(Pt 12), 2591-2598 (2004-05-26)
In animal cells, the endoplasmic reticulum may participate in programmed cell death by sensing and transducing apoptotic signals. In an attempt to analyze the role of the endoplasmic reticulum in plant programmed cell death we investigated the effect of cyclopiazonic

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