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APT173

Sigma-Aldrich

Caspase 9 Colorimetric Activity Assay Kit, LEHD

Caspase-9 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the LEHD.

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Synonym(s):
Mch-6, ICE-LAP6, Apaf-3
eCl@ss:
32161000

Quality Level

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable

NCBI accession no.

UniProt accession no.

detection method

colorimetric

shipped in

dry ice

Gene Information

human ... CASP9(842)

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APT139APT131APT165
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

Gene Information

human ... CASP9(842)

Gene Information

human ... CASP9(842)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

NCBI accession no.

NM_001229.2, NM_032996.1

NCBI accession no.

NM_001229.2, NM_032996.1

NCBI accession no.

NM_004346.3, NM_032991.2

NCBI accession no.

NM_004346.3, NM_032991.2

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.

Application

Caspase-9 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the LEHD.
Research Category
Apoptosis & Cancer

Components

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-9 Substrate (Ac-LEHD-pNA) (Part No. 90084): 1 mL of 3 mg/mL solution

Caspase-9 Inhibitor (Ac-LEHD-CHO) (Part No. 90087): 50 μL of 100 μM (0.054 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

Storage and Stability

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-9 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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