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C7729

Sigma-Aldrich

Anti-Caspase 9 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-APAF-3, Anti-APAF3, Anti-ICE-LAP6, Anti-MCH6, Anti-PPP1R56
MDL number:

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35-47 kDa (two bands)

species reactivity

human, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using microwave-treated tissue sections of human and rat heart
immunoprecipitation (IP): 10 μg using HeLa mitochondrial RIPA lysate (500 μg)
microarray: suitable
western blot: 1:300 using whole extract of human Jurkat cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP9(842)
rat ... Casp9(58918)

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This Item
C4356SAB5700630SAB5700785
biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

UniProt accession no.

P55211

UniProt accession no.

P55211

UniProt accession no.

P55211

UniProt accession no.

P55211

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General description

Anti-Caspase 9 is developed in rabbit using a synthetic peptide corresponding to amino acid of human procaspase 9 with N-terminal added lysine conjugated to keyhole limpet hemocyanin (KLH) with glutaraldehyde. Caspase 9/CASP9 (Mch6, ICE-LAP6, APAF3) is a member of the caspase 2 subfamily and is classified together with caspases 8 and 10 as an initiator caspase. It is a cysteine containing aspartate specific protease, made up of 416 amino acids, of which 13 are cysteine. CASP9 is located on human chromosome 1p36.21.

Immunogen

synthetic peptide corresponding to amino acid residues 299-316 of human procaspase 9 with N-terminal added lysine conjugated to KLH with glutaraldehyde.

Application

Anti-Caspase 9 antibody produced in rabbit has been used in western blot analysis.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Caspase 9/CASP9 is essential for apoptosis during normal development of the murine central nervous system and plays a role in controlling tumor development. It functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 can also be activated by granzyme B.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Dominika Karolczak et al.
Polish journal of pathology : official journal of the Polish Society of Pathologists, 64(3), 196-203 (2013-10-30)
Aging is the process of progressive accumulation of changes over time, which is additionally connected with increasing susceptibility to some diseases and ultimately leads to death. Aging is associated mainly with loss of permanent cells, e.g. in heart, skeletal muscle
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Lan A, et al.
Apoptosis, 12(3), 573-591 (2007)
Alessandro de Souza Prestes et al.
Toxicology in vitro : an international journal published in association with BIBRA, 55, 33-42 (2018-11-18)
Methylglyoxal (MG) is a α-dycarbonyl compound derived mainly from glycolysis, whose accumulation is harmful for cells and tissues. Here, we evaluated the cytotoxic effects induced by MG in leukocytes after an acute exposure, measuring as endpoints of toxicity some markers
Natalia Edison et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 18(9), 2569-2578 (2012-03-07)
XIAP [X-linked inhibitor of apoptosis (IAP) protein] is the best characterized mammalian caspase inhibitor. XIAP is frequently overexpressed in a variety of human tumors, and genetic inactivation of XIAP in mice protects against lymphoma. Therefore, XIAP is an attractive target
Analyses of apoptotic regulators CASP9 and DFFA at 1P36. 2, reveal rare allele variants in human neuroblastoma tumours
Abel F, et al.
British Journal of Cancer, 86(4), 596-596 (2002)

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