Skip to Content
MilliporeSigma
All Photos(5)

Documents

ABC495

Sigma-Aldrich

Anti-Caspase 3 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, Caspase 3

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

nonhuman primates (based on 100% sequence homology), feline (based on 100% sequence homology), canine (based on 100% sequence homology), rat (based on 100% sequence homology), hamster (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)

General description

Caspase-3 (EC 3.4.22.56; UniProt P42574; also known as Apopain, CASP-3, CPP-32, PARP cleavage protease, SCA-1, SREBP cleavage activity 1, Caspase 3 apoptosis-related cysteine protease, Cysteine protease CPP32, Procaspase3, Protein Yama) is encoded by the CASP3 (also known as CPP32, CPP32B) gene (Gene ID 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is initially produced with a propeptide sequence (a.a. 1-9), the removal of which yields the 268 a.a. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (a.a. 10-175) fragment and the p12 (a.a. 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (a.a. 29-175) subunit. The p17 and p12 dimerizes and forms the active caspase-3 enzyme. Caspase-3 plays a central role in the sequential activation of caspases during the execution phase of cell apoptosis. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer′s disease.

Specificity

Expected to react with pro-caspase 3, the uncleaved/inactive caspase 3 (32 kDa), and the p12 (12 kDa), but not the p17 (17 kDa) subunit of cleaved/active caspase 3.

Immunogen

Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Caspase 3 near the C-terminus.

Application

Anti-Caspase 3 Antibody is an antibody against Caspase 3 Antibody for use in Western Blotting and Immunocytochemistry.
Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Caspase 3 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Caspase 3 in 10 µg of A431 cell lysate.

Target description

~32 kDa observed

Linkage

Replaces: 06-375

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Tianxi Wang et al.
Experimental and therapeutic medicine, 21(6), 654-654 (2021-05-11)
Gastric carcinoma is a common type of gastrointestinal tumor with high morbidity and mortality rates. IL-17 is a newly discovered cytokine that has been reported to serve an important role in the development of gastric carcinoma. The potential effect of
Duojie Zhu et al.
Medical oncology (Northwood, London, England), 40(6), 182-182 (2023-05-19)
An increasing amount of evidence has demonstrated the anticancer activity of triterpenes extracted from traditional medicines. Echinocystic acid (EA), a natural triterpene isolated from Eclipta prostrata (L.) L., has previously been shown to exhibit anticancer activity in HepG2 and HL-60

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service