MilliporeSigma
All Photos(1)

Documents

SAB4200714

Sigma-Aldrich

Anti-Heat Shock Protein 70 (HSP70) antibody, Mouse monoclonal

clone BRM-22, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-HSP70, Anti-HSPA1A
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

Quality Level

antibody product type

primary antibodies

clone

BRM-22, monoclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse

concentration

~1 mg/mL

technique(s)

ELISA: suitable
dot blot: suitable
immunoblotting: 0.25-0.5 μg/mL using HeLa cell line extract
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPA1A(3303)
mouse ... HSPA1A(193740)
rat ... HSPA1A(24472)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
H5147SAB4200797H1775
antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

clone

BRM-22, monoclonal

clone

BRM-22, monoclonal

clone

polyclonal

clone

AC-16, monoclonal

species reactivity

plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse

species reactivity

bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster

species reactivity

chicken, rat, canine, human, monkey, mouse, bovine

species reactivity

wheat germ, chicken, Sf9 cell line, rabbit, rat, human, mouse, Achlya

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P0DMV8

UniProt accession no.

P0DMV8

UniProt accession no.

P0DMV8

UniProt accession no.

P07900

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Anti-heat shock protein 70 (HSP70) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the BRM-22 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified bovine brain HSP70. Monoclonal Anti-heat shock protein 70 (HSP70) specifically recognizes HSP70 from bovine, mouse, monkey, dog, human, rat, chicken,octopus, insects, plant, fish, rabbit and sea urchin origin.
HSP70 / heat shock protein family A (Hsp70) member 1A (HSPA1A), is encoded by the gene mapped to human chromosome 6p21.33. It is widely expressed in all tissues. The encoded protein is characterized with an N-terminal ATPase domain of 45kDa and a C-terminal substrate binding domain of 25kDa which is further subdivided into a β-sandwich subdomain of 15kDa and a C-terminal α-helical subdomain.

Immunogen

purified bovine brain HSP70

Biochem/physiol Actions

The members of Hsp70 protein family are implicated in various cellular processes and are required for the response to environmental changes and survival. These proteins are essential constituents of the cellular network of molecular chaperones and folding catalysts. Hsp70 plays a vital role in several folding processes, such as the folding and assembly of newly synthesized proteins, refolding of misfolded and aggregated proteins. In addition, it also facilitates membrane translocation of organellar and secretory proteins and regulate the activity of regulatory proteins. Aberrations in the gene expression leads to skin color diversity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Variation in Hsp70-1A expression contributes to skin color diversity
Murase D, et al.
The Journal of Investigative Dermatology, 136, 1681-1691 (2016)
Honghong Zhang et al.
Oncology letters, 17(6), 5601-5607 (2019-06-13)
To the best of our knowledge, the microRNA (miR/miRNA) expression profile of plasma exosomes in ovarian cancer has not been previously studied. The aim of the present study was to investigate the practicality of using plasma exosomal miRNAs as novel
Dániel J Kócsó et al.
Mycotoxin research, 37(2), 141-148 (2021-03-06)
The heat shock protein (Hsp70) level was assessed after 14 days of oral gavage-exposure to fumonisin B1 (FB1: 150 µg/animal/day), deoxynivalenol (DON: 30 µg/animal/day) and zearalenone (ZEN: 150 µg/animal/day), alone or in combinations (in additive manner: FD = FB1 + DON, FZ = FB1 + ZEN, DZ = DON + ZEN and FDZ = FB1 + DON + ZEN) in the liver
hsp70 genes in the human genome: Conservation and differentiation patterns predict a wide array of overlapping and specialized functions
Brocchieri L, et al.
BMC Evolutionary Biology (2008)
Hsp70 chaperones: Cellular functions and molecular mechanism
Mayer MP and Bukau B.
Cellular and Molecular Life Sciences, 62, 670-684 (2005)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service