ZRB1239

Anti-GRP-78 Antibody, clone 1B11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): 78 kDa glucose-regulated protein, BiP, Binding-immunoglobulin protein, EC:3.6.4.10, Endoplasmic reticulum chaperone BiP, HSP70 family protein 5, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B11, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 72.33 kDa; observed mol wt ~72 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): canine, monkey, bovine, porcine, feline
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_005338.1
• UniProt accession no.: P11021
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... GRP78(3309)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects 78 kDa glucose-regulated protein (GRP-78). It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human 78 kDa glucose-regulated protein (GRP-78).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GRP-78 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GRP-78 in lysates from U87-MG, RAW264.7, and NIH3T3 cells and recombinant full-length Human GRP-78 (without the signal peptide).

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GRP-78 in human liver tissue sections.

Affinity Binding Assay Analysis: A representative lot of this antibody bound GRP-78 peptide with a KD of 9.8 x 10-6 in an affinity binding assay..

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Endoplasmic reticulum chaperone BiP (UniProt: P11021; also known as EC:3.6.4.10, 78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, Heat shock protein 70 family protein 5, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein) is encoded by the HSPA5 (also known as GRP78) gene (Gene ID: 3309) in human. GRP-78 is a multifunctional, abundant protein of the Hsp70 family. However, unlike the other members of this family, GRP-78 is not induced by heat shock because the promoter of GRP78 lacks the heat shock element. It is maintained at relatively low levels within the cell and its level increases significantly under stress condition that affect the endoplasmic reticulum (ER) and calcium homeostasis. It serves as an ER chaperone and exists either as a monomer or as a homooligomer. It plays a key role in protein folding and quality control in ER lumen. It is involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5 and also acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains a nucleotide-binding domain (NBD; aa 125-280), a substrate-binding domain (aa 420-500), and a short interdomain linker (aa 409-419). The interdomain linker regulates its chaperone activity by mediating it homo-oligomerization. Its chaperone activity is regulated by ATP-induced allosteric coupling of the NBD and substrate-binding domains. GRP78 expression is shown to be up-regulated during early cardiogenesis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Casas, C. (2017). Front. Neurosci. 11; 177; Leung, HW., et al. (2015). J. Mol. Cell. Cardiol. 82; 228-237; Ng, DT., et al. (1992). Mol. Biol. Cell. 3(2); 143-155).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable