MilliporeSigma
All Photos(5)

Documents

X2252

Sigma-Aldrich

Xanthine Oxidase microbial

lyophilized powder, ≥7 units/mg solid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

microbial

Quality Level

form

lyophilized powder

specific activity

≥7 units/mg solid

mol wt

~160 kDa

storage condition

(Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons)

technique(s)

inhibition assay: suitable

color

brown

optimum pH

7.5-8.0

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
X4500SAE0044X1875
Xanthine Oxidase microbial lyophilized powder, ≥7 units/mg solid

X2252

Xanthine Oxidase microbial

Xanthine Oxidase from bovine milk Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

X4500

Xanthine Oxidase from bovine milk

Sigma-Aldrich

X1875

Xanthine Oxidase from bovine milk

Gene Information

Escherichia coli K12 ... xdhA(947116), xdhB(947205), xdhC(945148)

Gene Information

-

Gene Information

-

Gene Information

-

specific activity

≥7 units/mg solid

specific activity

≥0.8 units/mg protein

specific activity

≥12 units/mg protein

specific activity

≥0.4 units/mg protein

biological source

microbial

biological source

bovine milk

biological source

bacterial (arthrobacter sp.)

biological source

-

form

lyophilized powder

form

ammonium sulfate suspension

form

powder

form

ammonium sulfate suspension

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

General description

Research area: Cell Cycle

Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Application

Xanthine Oxidase microbial has been used:
  • to determine in vitro xanthine oxidase activity
  • as a source to generate superoxide anions during superoxide dismutase assay
  • as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction

Biochem/physiol Actions

Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.
Also contains proteolytic activity.

Physical properties

Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Lyophilized powder containing BSA and sodium glutamate as stabilizers

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Xanthine ≥99.5% (HPLC), purified by recrystallization

Sigma-Aldrich

X0626

Xanthine

Xanthine BioUltra, ≥99%

Sigma-Aldrich

X4002

Xanthine

Xanthine sodium salt ≥99%

Sigma-Aldrich

X2502

Xanthine sodium salt

Allopurinol xanthine oxidase inhibitor

Sigma-Aldrich

A8003

Allopurinol

Pál Pacher et al.
Pharmacological reviews, 58(1), 87-114 (2006-03-02)
The prototypical xanthine oxidase (XO) inhibitor allopurinol, has been the cornerstone of the clinical management of gout and conditions associated with hyperuricemia for several decades. More recent data indicate that XO also plays an important role in various forms of
Michael C Hall
Molecular and cellular biochemistry, 292(1-2), 1-11 (2006-09-20)
The cytotoxicity of five oxysterols identified in cooked fish, 7-ketocholesterol, 7beta-hydroxycholesterol, cholesterol 5alpha,6alpha-epoxide, cholestanetriol and 4-cholesten-3-one, was investigated in primary cultures of bovine ovarian granulosa cells. Cells were exposed to the oxysterols individually and to a mixture of the same
P J Marro et al.
Pediatric research, 41(4 Pt 1), 513-520 (1997-04-01)
The effect of allopurinol to inhibit purine metabolism via the xanthine oxidase pathway in neonates with severe, progressive hypoxemia during rescue and reperfusion with extracorporeal membrane oxygenation (ECMO) was examined. Twenty-five term infants meeting ECMO criteria were randomized in a
Giuseppe Faudone et al.
Journal of medicinal chemistry, 64(11), 7156-7178 (2021-05-22)
The purine alkaloid caffeine is the most widely consumed psychostimulant drug in the world and has multiple beneficial pharmacological activities, for example, in neurodegenerative diseases. However, despite being an extensively studied bioactive natural product, the mechanistic understanding of caffeine's pharmacological
COLORIMETRIC PAPER-BASED DEVICE BY ENZYMATIC REACTION FOR DETECTING ALLOPURINOL IN TRADITIONAL MEDICINE
Rimadani P, et al.
Rasayan Journal of Chemistry (2021)

Protocols

The appearance of uric acid is measured at 293nm by spectrophotometry.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service