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10102121001

Roche

Adenosine Deaminase (ADA)

from calf intestine

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Synonym(s):
ADA, Adenosine Deaminase
Enzyme Commission number:

biological source

bovine intestine

Quality Level

form

suspension

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

packaging

pkg of 1 mL (10 mg)

manufacturer/tradename

Roche

technique(s)

activity assay: suitable

color

white

optimum pH

7.0-7.4

solubility

water: miscible

suitability

suitable for chemiluminescence

NCBI accession no.

UniProt accession no.

application(s)

life science and biopharma

foreign activity

AMP-deaminase <0.01%
AP <0.01% (with pre incubation)
Guanase <0.01%
NP <0.01%

storage temp.

2-8°C

Gene Information

bovine ... ADA(280712)

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This Item
10102105001A5168A5043
vibrant-m

10102121001

Adenosine Deaminase (ADA)

vibrant-m

10102105001

Adenosine Deaminase (ADA)

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Gene Information

bovine ... ADA(280712)

Gene Information

-

Gene Information

cow ... ADA(280712)

Gene Information

cow ... ADA(280712)

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

specific activity

150-200 units/mg protein

specific activity

≥130 units/mg protein

NCBI accession no.

AAF91430

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

-

color

white

color

-

color

-

color

-

General description

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Chemiluminescent adenosine assay.
Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.

Biochem/physiol Actions

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


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Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but

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