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A5168

Sigma-Aldrich

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

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Synonym(s):
Adenosine aminohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bovine spleen

Quality Level

type

Type IX

form

ammonium sulfate suspension

specific activity

150-200 units/mg protein

mol wt

32.5-33 kDa

UniProt accession no.

foreign activity

5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Gene Information

cow ... ADA(280712)

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This Item
A5043S8409L2625
specific activity

150-200 units/mg protein

specific activity

≥130 units/mg protein

specific activity

2,000-6,000 units/mg protein (biuret)

specific activity

≥500 units/mg protein

biological source

bovine spleen

biological source

bovine spleen

biological source

-

biological source

-

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

ammonium sulfate suspension

UniProt accession no.

P56658

UniProt accession no.

P56658

UniProt accession no.

P00442

UniProt accession no.

P19858, Q5E9B1

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.
Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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White adipose tissue (WAT) and brown adipose tissue (BAT) play distinct roles in adaptation to changes in nutrient availability, with WAT serving as an energy store and BAT regulating thermogenesis. We previously showed that mice maintained on a leucine-deficient diet
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Adenosine deaminase is an enzyme of the purine metabolism whose function is to convert adenosine to inosine and deoxyadenosine to deoxyinosine. The ecto-ADA1 binding to the cell surface through CD26 contributes to the regulation of cytokines and stimulates the proliferation

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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