MilliporeSigma
All Photos(5)

Documents

H9377

Sigma-Aldrich

Hypoxanthine

≥99.0%

Sign Into View Organizational & Contract Pricing

Synonym(s):
6-Hydroxypurine
Empirical Formula (Hill Notation):
C5H4N4O
CAS Number:
Molecular Weight:
136.11
Beilstein/REAXYS Number:
5811
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.51

biological source

synthetic (organic)

Quality Level

assay

≥99.0%

form

powder

mp

>300 °C (lit.)

solubility

formic acid: water (2:1): 50 mg/mL, clear to slightly hazy, colorless to yellow

SMILES string

O=C1NC=Nc2nc[nH]c12

InChI

1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)

InChI key

FDGQSTZJBFJUBT-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
H9636Y0000441Y0002147
Hypoxanthine ≥99.0%

H9377

Hypoxanthine

Hypoxanthine powder, BioReagent, suitable for cell culture

H9636

Hypoxanthine

Didanosine impurity A, European Pharmacopoeia (EP) Reference Standard

Y0000441

Didanosine impurity A,

Mercaptopurine impurity A CRS, European Pharmacopoeia (EP) Reference Standard

Y0002147

Mercaptopurine impurity A

Quality Level

300

Quality Level

-

Quality Level

-

Quality Level

-

form

powder

form

powder

form

-

form

-

assay

≥99.0%

assay

≥99%

assay

-

assay

-

solubility

formic acid: water (2:1): 50 mg/mL, clear to slightly hazy, colorless to yellow

solubility

formic acid: water (2:1): 50 mg/mL

solubility

-

solubility

-

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
  • to gavage mice and to evaluate the activity of xanthine oxidase (XO) in pasteurized whole milk in vivo in potassium oxonate (PO) -induced mouse model of hyperuricemia
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) medium to cultivate in-vitro cell line (EL-1 cells)
  • as a pure standard for the quantification of hypoxanthine in vitreous fluid sample using high-performance liquid chromatography-ultra-violet (HPLC-UV) for the estimation of post-mortem interval

Biochem/physiol Actions

Hypoxanthine acts as an intermediate in purine metabolism. It elicits a vital role as a 5′-base of the anticodon in a few transfer-RNAs. Though similar to guanine (Gua), it is devoid of the exocyclic amino group at C(2) position. It may be used as a substrate to study the specificity and kinetics of hypoxanthine-guanine phosphoribosyl transferases.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

Thymidine ≥99%

Sigma-Aldrich

T9250

Thymidine

Guanosine ≥98%

Sigma-Aldrich

G6752

Guanosine

Thymidine powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T1895

Thymidine

Xanthine BioUltra, ≥99%

Sigma-Aldrich

X4002

Xanthine

Influence of the nature of death in biochemical analysis of the vitreous humour for the estimation of post-mortem interval
Perez-Martinez C, et al.
Australian journal of public health (2020)
Dhaksshaginy Rajalingam et al.
International archives of occupational and environmental health, 94(8), 1905-1915 (2021-06-03)
Several studies show that severe social stressors, e.g., in the form of exposure to workplace bullying in humans, is associated with negative mental health effects such as depression and anxiety among those targeted. However, the understanding of the underlying biological
Ultrafast electronic deactivation dynamics of the rare natural nucleobase hypoxanthine
Rottger K, et al.
Chemical Physics Letters (2012)
Different processed milk with residual xanthine oxidase activity and risk of increasing serum uric acid level
Sha W, et al.
Food Bioscience (2021)
Javier Acosta et al.
Frontiers in bioengineering and biotechnology, 8, 677-677 (2020-07-17)
In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT/AMPK). In this respect, we report for the first time

Articles

Uric acid is not a direct additive to any of the classical cell culture media. However, it is present in animal sera. Consequently, uric acid is typically present in complete serum-supplemented classical cell culture systems.

Primary cell culture tips: Video segments offer guidance on primary cell culture, emphasizing physiological relevance and careful handling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service