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T1895

Sigma-Aldrich

Thymidine

powder, BioReagent, suitable for cell culture

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Synonym(s):
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
Empirical Formula (Hill Notation):
C10H14N2O5
CAS Number:
Molecular Weight:
242.23
Beilstein:
89285
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic (organic)

Quality Level

product line

BioReagent

Assay

≥99%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

186-188 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless

SMILES string

CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O

InChI

1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1

InChI key

IQFYYKKMVGJFEH-XLPZGREQSA-N

Gene Information

human ... DTYMK(1841)

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1 of 4

This Item
T9250PHR316389270
Thymidine powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T1895

Thymidine

Thymidine ≥99%

Sigma-Aldrich

T9250

Thymidine

Thymidine ≥99.0% (HPLC)

Sigma-Aldrich

89270

Thymidine

assay

≥99%

assay

≥99%

assay

-

assay

≥99.0% (HPLC)

form

powder

form

powder

form

-

form

powder

mp

186-188 °C (lit.)

mp

186-188 °C (lit.)

mp

186-188 °C (lit.)

mp

185-190 °C, 186-188 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless

solubility

water: 50 mg/mL, clear, colorless

solubility

-

solubility

hot water: 1 g/10 mL

product line

BioReagent

product line

-

product line

-

product line

-

General description

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.

Application

Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
  • as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
  • as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
  • to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells

Biochem/physiol Actions

Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Optimization in Drug Discovery: In Vitro Methods (2016)
Serum neurogranin measurement as a biomarker of acute traumatic brain injury
Yang J, et al.
Clinical Biochemistry, 43(13-14), 843-848 (2015)
Human Biochemistry and Disease, 571-571 (2008)
Molecular Biology, Principles of Genome Function: Biology, Molecular biology (2016)
Dominique Desbouis et al.
Journal of medicinal chemistry, 51(21), 6689-6698 (2008-10-08)
Human cytosolic thymidine kinase (hTK1) has proven to be a suitable target for noninvasive imaging of cancer cell proliferation using radiolabeled substrates such as [ (18)F]fluorothymidine ([ (18)F]FLT). However, a thymidine tracer useful for single photon emission tomography (SPECT) based

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