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MAK466

Sigma-Aldrich

Free Fatty Acid Assay Kit

new

sufficient for 100 colorimetric or fluorometric tests

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Quality Level

application(s)

agriculture
food and beverages
pharmaceutical

detection method

colorimetric
fluorometric

relevant disease(s)

diabetes; pediatric diseases; endocrinological disorders; neurological disorders

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Fatty acids are aliphatic monocarboxylic acids ubiquitously found in animal or vegetable fat, oil, and wax. Fatty acids play important roles in cellular synthesis, energy metabolism, and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome, and Reye Syndrome.

Features and Benefits

  • Linear detection range: colorimetric assay 7 - 1000 µM, fluorometric assay 7 - 100 µM fatty acid.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for detecting free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, and cell cultures, and in food and agriculture products, as well as studying the effects of drugs on free fatty acid metabolism.

Principle

The Free Fatty Acid Assay Kit provides a simple, one-step and high-throughput assay for measuring Free Fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity (λEx = 530 nm/λEm = 585 nm) is directly proportional to the free fatty acid concentration in the sample.

pictograms

CorrosionEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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25G
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MMYOMAG-74K-13

1000309185

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