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TR0100

Sigma-Aldrich

Serum Triglyceride Determination Kit

1 kit sufficient for 250 tests

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Synonym(s):
Triglyceride and Free Glycerol Kits and Reagents
NACRES:
NA.26

Quality Level

usage

 kit sufficient for 250 tests

storage temp.

2-8°C

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This Item
FG0100CGD1MAK045
Free Glycerol Determination Kit 1 kit sufficient for 1000 reactions

FG0100

Free Glycerol Determination Kit

HDL and LDL/VLDL Quantitation Kit sufficient for 100 colorimetric or fluorometric tests

MAK045

HDL and LDL/VLDL Quantitation Kit

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

The Serum Triglyceride Determination Kit is for the quantitative enzymatic measurement of glycerol, true triglycerides, and total triglycerides in serum or plasma at 540 nm. Triglycerides, esters of fatty acids and glycerol,1 do not circulate freely in plasma, but are bound to proteins and transported as macromolecular complexes called lipoproteins.2 Methods for triglyceride determination generally involve enzymatic3 or alkaline4 hydrolysis of triglycerides to glycerol and free fatty acids, followed by either chemical or enzymatic measurement of the glycerol released.

Application

A Serum Triglyceride Determination Kit was used to determine the level of triglycerides in whole-body homogenates of Drosophila melanogaster.
The Serum Triglyceride Determination Kit can be used for the measurement of glycerol, true triglycerides, or total triglycerides in serum or plasma. The procedure involves enzymatic hydrolysis by lipase of the triglycerides to glycerol and free fatty acids. The glycerol produced is then measured by coupled enzyme reactions. Many of the triglyceride reagents which are commercially available, do not differentiate between endogenous glycerol and glycerol derived by hydrolytic action of lipase on glycerides.
Triglycerides are first hydrolyzed by lipoprotein lipase to glycerol and free fatty acids. Glycerol is then phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxy-acetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase (POD) catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium
N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample.
The triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides.

Packaging

The kit also includes sufficient reagent for an additional 250 free glyceride tests for true triglyceride determination.

Suitability

Suitable for the quantitative determination of glycerol, total triglycerides or free triglycerides in serum or plasma

Principle

The Serum Triglyceride Determination Kit can be used for the measurement of glycerol, true triglycerides, or total triglycerides in serum or plasma. The procedure involves enzymatic hydrolysis by lipase of the triglycerides to glycerol and free fatty acids. The glycerol produced is then measured by coupled enzyme reactions. Many of the triglyceride reagents which are commercially available, do not differentiate between endogenous glycerol and glycerol derived by hydrolytic action of lipase on glycerides. Triglycerides are first hydrolyzed by lipoprotein lipase to glycerol and free fatty acids. Glycerol is then phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxy-acetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase (POD) catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample.

Linkage

In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.

Kit Components Also Available Separately

Product No.
Description
SDS

  • F6428Free Glycerol Reagent, used for quantitative enzymatic determination of glycerol 10 x 40SDS

  • T2449Triglyceride Reagent, used for quantitative enzymatic determination of triglyerides 5 x 10SDS

standard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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