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MAK331

Sigma-Aldrich

AF HDL and LDL/VLDL Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
Total Cholesterol Assay Kit

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

pharmaceutical

detection method

colorimetric

relevant disease(s)

cardiovascular diseases

storage temp.

−20°C

Related Categories

General description

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable.

Application

The AF HDL and LDL/VLDL Assay Kit may be used for:
  • Cancer Research
  • Cardiovascular Research
  • Neurological Research

Features and Benefits

Efficient and Precise: Detect Cholesterol levels ranging from 1 to 100 mg/dL (colorimetric) and 0.2 to 10 mg/dL (fluorimetric) in a 30 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for HDL and LDL/VLDL cholesterol detection in serum samples and for the evaluation of drugs on cholesterol metabolism.

Principle

The AF HDL and LDL/VLDL Assay Kit is based on an improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorometric assays.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3


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