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MAK043

Sigma-Aldrich

Cholesterol Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
Cholesterol Assay Kit
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

cancer; cardiovascular diseases

storage temp.

−20°C

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This Item
MAK045MAK325CS0005
Cholesterol Quantitation Kit sufficient for 100 colorimetric or fluorometric tests

MAK043

Cholesterol Quantitation Kit

HDL and LDL/VLDL Quantitation Kit sufficient for 100 colorimetric or fluorometric tests

MAK045

HDL and LDL/VLDL Quantitation Kit

Cholesterol Uptake Assay Kit sufficient for 100 fluorometric tests

MAK325

Cholesterol Uptake Assay Kit

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

fluorometric

detection method

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer; cardiovascular diseases

relevant disease(s)

cardiovascular diseases

relevant disease(s)

obesity; cardiovascular diseases; cancer

relevant disease(s)

-

General description

Cholesterol is an important component of mammalian cell membranes where it functions in intracellular transport, cell signaling, and maintaining membrane fluidity. Within the blood, cholesterol circulates as both the free acid and as cholesterol esters. Controlling serum cholesterol has an important therapeutic role as elevated cholesterol levels are associated with the development of atherosclerosis and cardiovascular pathologies. Recent evidence suggests a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.

Cholesterol is predominantly produced in the liver. Abnormal cholesterol levels are implicated in coronary heart disease. Cholesterol is associated with breast cancer progression or inhibition. Tumor cells are capable of synthesizing their own cholesterol, which aids in cell proliferation. Increased cholesterol level is observed in cancers, especially in prostate cancer. Elevated serum cholesterol is observed in obesity.

Application

Cholesterol Quantitation Kit has been used to measure the total serum cholesterol level.

Suitability

Suitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples.

Principle

The Cholesterol Quantitation Kit can also be used to determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. In this kit, total cholesterol concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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Cholesterol and Cancer, in the Balance.
Sandrine S P and Poirot M
Science, 343(6178), 1445-1446 (2014)
Mingzhi Han et al.
EMBO molecular medicine, 12(1), e10924-e10924 (2019-11-30)
Dysregulated cholesterol metabolism is a hallmark of many cancers, including glioblastoma (GBM), but its role in disease progression is not well understood. Here, we identified cholesterol 24-hydroxylase (CYP46A1), a brain-specific enzyme responsible for the elimination of cholesterol through the conversion
Hypolipidemic and Anti-Atherogenic Activity of Aqueous Extract of Leaves of Lagenaria siceraria in Wistar Rats.
Aslam M and Nijam R
Journal of Natural Remedies, 14(1), 52-57 (2013)
Time trends in population cholesterol levels 1986--2004: influence of lipid-lowering drugs, obesity, smoking and educational level. The northern Sweden MONICA study
Eliasson M, et al.
J. Int. Med., 260(6), 551-559 (2006)
Is There Any Relationship between Diabetic Gastroparesis and Mucosal Injury?
Cakir O O.
J Gastrointest Dig Syst , 6(461), 2-2 (2016)

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