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MAK040

Sigma-Aldrich

Adipogenesis Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
Triglyceride Assay Kit
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cardiovascular diseases; cancer

storage temp.

−20°C

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This Item
MAK043SCR020C-27417
vibrant-m

MAK040

Adipogenesis Kit

vibrant-m

MAK043

Cholesterol Quantitation Kit

vibrant-m

SCR020

Mesenchymal Adipogenesis Kit

vibrant-m

C-27417

Preadipocyte Growth Medium

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

-

usage

-

detection method

colorimetric

detection method

colorimetric, fluorometric

detection method

-

detection method

-

relevant disease(s)

endocrinological disorders, diabetes; cardiovascular diseases; cancer

relevant disease(s)

cancer; cardiovascular diseases

relevant disease(s)

-

relevant disease(s)

-

General description

Adipogenesis is the differentiation of pre-adipocytes derived from pluripotent mesenchymal cells into mature adipocytes. Adipocytes, which are capable of storing large quantities of lipids as triglycerides, are the main cells for lipid storage in animals. Adipose tissue plays a critical role in the regulation of whole-body energy homeostasis and adipocyte dysfunction has been implicated in pathological states such as cancer, type II diabetes, cardiovascular disease, and atherosclerosis.

Omental adipocytes are known to promote the invasion and progression of cancer cells especially, ovarian cancer. Close proximity of tumor cells and adipocytes, causes a phenotypic change resulting in the formation of adipocyte-derived fibroblasts, which directly promotes tumor progression. Elevated level of adiponectin produced by adipocytes is observed in heart failure patients, cardiovascular disease, and atherosclerosis. Excess cytokines such as TNFα (tumor necrosis factor -α) in adipocytes results in chronic inflammatory state and inhibits the process of adipogenesis. Adipocytes are known to maintain glucose homeostasis. Adipocytes regulates the entry of fatty acids into circulation and skeletal muscle. This action of adipocytes is associated with the development of type 2 diabetes, which indicates insulin resistance as a result of high levels of fatty acids in the blood.

Application

Adipogenesis Kit has been used to study the intramyocellular triglyceride accumulation in myotubes. It has been used to study the effect of NS5ABP37 on hepatocellular carcinoma by determining the total intracellular triglyceride.

Suitability

Suitable for the quantification of triglyceride accumulation in cells and tissue

Principle

In this kit, total cellular and tissue concentrations of triglycerides are determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the triglycerides present. This kit is able to detect triglycerides in as few as 1 × 104 differentiated 3T3 L1 cells.

pictograms

CorrosionHealth hazard

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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A new role for the natriuretic peptides: metabolic regulators of the adipocyte.
Costello-Boerrigter L C and Burnett J C
Journal of the American College of Cardiology (2009)
Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
Nieman K M, et al.
Nature Medicine, 17(11), 1498-1498 (2011)
Xubin Lu et al.
The Journal of dairy research, 87(2), 232-238 (2020-04-17)
In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis
Yufei Zheng et al.
Nutrients, 12(4) (2020-04-03)
The increasing incidence of obesity poses a great threat to public health worldwide. Recent reports also indicate the relevance of obesity in metabolic diseases. Chinese propolis (CP), as a well-studied natural nutraceutical, has shown a beneficial effect on alleviating diabetes
Defining the role of DAG, mitochondrial function and lipid deposition in palmitate-induced proinflammatory signalling and its counter-modulation by palmitoleate.
Macrae K, et al.
Journal of Lipid Research, M036996-M036996 (2013)

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