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MAK313

Sigma-Aldrich

Adipolysis Assay Kit

sufficient for 200 colorimetric or fluorometric tests

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NACRES:
NA.84

application(s)

pharmaceutical

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; obesity; cardiovascular diseases; cancer

storage temp.

−20°C

Related Categories

General description

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for adipolysis quantification in cell culture.

Principle

This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step, resulting in a colorimetric (570 nm)/fluorimetric (λex = 530/ λem = 587 nm) product, proportional to the glycerol present.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F

flash_point_c

87 °C


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