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M4655

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
EMEM, MEM
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
M2414FG0325M0325
Sigma-Aldrich

M4655

Minimum Essential Medium Eagle

Sigma-Aldrich

M2414

Minimum Essential Medium Eagle

SAFC

FG0325

Minimum Essential Medium Eagle

Sigma-Aldrich

M0325

Minimum Essential Medium Eagle

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

components

phenol red: 0.011 g/L
NaHCO3: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no

components

stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no
Hanks’ salts (2% CO2): no
glucose: yes
NaHCO3: yes

components

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO2): yes
NaHCO3: 2.2 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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