SAB4200747

Anti-Neurofilament 200 antibody, Mouse monoclonal

clone NE14, purified from hybridoma cell culture

• Synonym(s): Anti-H-subunit, Anti-NF-H
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NE14, monoclonal
• form: buffered aqueous solution
• mol wt: ~200 kDa
• species reactivity: feline, rat, guinea pig, pig, human, mouse, bovine, chicken
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction; immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections
• isotype: IgG1
• UniProt accession no.: P12036
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... Nefh(528842) ; cat ... Nefh(101087272) ; chicken ... Nefh(417020) ; human ... NEFH(4744) ; mouse ... Nefh(380684) ; pig ... Nefh(100156492) ; rat ... Nefh(24587)

Description

General description

Anti-Neurofilament 200 Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NE14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as neurofilament heavy polypeptide (Hsubunit), NF-H, NEFH or 200 kDa neurofilament protein.

Immunogen

Neurofilaments purified from pig spinal cord

Application

Anti-Neurofilament 200 antibody, Mouse monoclonal has been used in immunoblotting and immunohistochemistry staining.

Biochem/physiol Actions

Neurofilaments are the type of intermediate filaments (IFs), that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. It has an important function in mature axons that is not sub served by the two smaller neurofilament proteins. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion. Neurofilaments can accumulate in large numbers within cell bodies and proximal axons of affected neurons in several pathological diseases, such as Charcot-Marie-Tooth (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, neurofilament accumulations was detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable