N5139
Monoclonal Anti-Neurofilament 68 antibody produced in mouse
clone NR4, ascites fluid
Synonym(s):
Monoclonal Anti-Neurofilament light chain
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NR4, monoclonal
mol wt
antigen apparent mol wt 68 kDa
contains
15 mM sodium azide as preservative
species reactivity
chicken, rat, pig, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using rat cerebellum
microarray: suitable
western blot: 1:500 using bovine spinal cord neurofilament
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NEFL(4747)
General description
Monclonal Anti-Neurofilament 68 (mouse IgG1 isotype) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins. The neurofilaments are one of the five major groups of IFs and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 95kD, and 115kD. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals.
Specificity
The antibody reacts specifically with neurofilament 68 in cultured cells or tissue preparations originating from human, pig, rat or chicken. It does not cross react with other intermediate filament proteins.
Immunogen
pig spinal cord.
Application
Monoclonal Anti-Neurofilament 68 antibody has been used:
- in immunohistochemistry
- in immunoblotting
- in western blotting
Mouse Monoclonal clone NR4 anti-Neurofilament 68 antibody may be used for the localization of the neurofilament of molecular weight equal to 68,000 while being non-reactive with other intermediate filament proteins.
Biochem/physiol Actions
Neurofilaments undergo post-translational modification, which results in their heterogeneity, including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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