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N5139

Sigma-Aldrich

Monoclonal Anti-Neurofilament 68 antibody produced in mouse

clone NR4, ascites fluid

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Synonym(s):
Monoclonal Anti-Neurofilament light chain
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NR4, monoclonal

mol wt

antigen apparent mol wt 68 kDa

contains

15 mM sodium azide as preservative

species reactivity

chicken, rat, pig, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using rat cerebellum
microarray: suitable
western blot: 1:500 using bovine spinal cord neurofilament

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NEFL(4747)

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This Item
N5264N0142N2912
Gene Information

human ... NEFL(4747)

Gene Information

chicken ... NEFM(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

Gene Information

human ... NEFH(4744), NEFM(4741)
mouse ... Nefh(380684), Nefm(18040)
rat ... Nefh(24587), Nefm(24588)

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

chicken, rat, pig, human

species reactivity

human, pig

species reactivity

wide range

species reactivity

mouse, rat, human

clone

NR4, monoclonal

clone

NN18, monoclonal

clone

N52, monoclonal

clone

RMdO20, monoclonal

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General description

Monclonal Anti-Neurofilament 68 (mouse IgG1 isotype) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins. The neurofilaments are one of the five major groups of IFs and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 95kD, and 115kD. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals.

Specificity

The antibody reacts specifically with neurofilament 68 in cultured cells or tissue preparations originating from human, pig, rat or chicken. It does not cross react with other intermediate filament proteins.

Immunogen

pig spinal cord.

Application

Monoclonal Anti-Neurofilament 68 antibody has been used:
  • in immunohistochemistry
  • in immunoblotting
  • in western blotting

Mouse Monoclonal clone NR4 anti-Neurofilament 68 antibody may be used for the localization of the neurofilament of molecular weight equal to 68,000 while being non-reactive with other intermediate filament proteins.

Biochem/physiol Actions

Neurofilaments undergo post-translational modification, which results in their heterogeneity, including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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