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C8035

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Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse

clone CS-56, ascites fluid

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Synonym(s):
Chondroitin Sulfate Antibody, Chondroitin Sulfate Antibody - Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse, Cs56 Antibody
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CS-56, monoclonal

species reactivity

chicken, bovine, mouse, human, pig, rabbit, rat

technique(s)

indirect immunofluorescence: 1:200 using bovine mammary gland epithelial (BMGE) cells
microarray: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)
mouse ... Acan(11595)
rat ... Acan(58968)

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This Item
SAB4200696D8168S5768
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

chicken, bovine, mouse, human, pig, rabbit, rat

species reactivity

mouse, chicken, bovine, human

species reactivity

chicken, rat, human, pig, rabbit, mouse

species reactivity

pig, rat, human, guinea pig

clone

CS-56, monoclonal

clone

CS-56, monoclonal

clone

MANDYS8, monoclonal

clone

SVP-38, monoclonal

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

Gene Information

human ... ACAN(176)
mouse ... Acan(11595)
rat ... Acan(58968)

Gene Information

human ... CSPG4(1464)

Gene Information

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

Gene Information

human ... SYP(6855)
rat ... Syp(24804)

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General description

Chondroitin sulfate is a linear polysaccharide composed of alternating disaccharide glucuronic acid and N-acetylgalactosamine. It contains proteoglycan (PG) of the extracellular matrix (ECM). Monoclonal Anti-Chondroitin Sulfate (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
This product can be found as purified product that was produced using cell culture hybridoma (SAB4200696)

Specificity

The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.

Immunogen

Ventral membranes of chicken gizzard fibroblasts

Application

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse was used:
  • in immunohistochemistry as primary antibody to study of spinal cords and brain cryosections.
  • in ELISA and western blot for purification of soluble forms of myelin-associated glycoprotein (MAG) and neural cell adhesion molecule (N-CAM).
  • in flow cytometry studies.
  • as primary antisera in a study of TGF-β regulation.
Monoclonal Anti-Chondroitin Sulfate can also be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts. It is also suitable for microarray and indirect immunofluorescence at the dilution of 1:200 by using bovine mammary gland epithelial (BMGE) cells.

Biochem/physiol Actions

Chondroitin sulfate is involved in tumor invasion and also plays a vital role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. In Chondroitin sulfate proteoglycan (CSPG), proteoglycan part is involved in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. Chondroitin sulfate is used in osteoarthritis treatment. Upregulation of chondroitin sulfate proteoglycans is observed after neuronal injury. It possess the ability to inhibit axonal regeneration.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200696 Anti-Chondroitin Sulfate antibody, Mouse monoclonal
clone CS-56, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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PTPσ is a receptor for chondroitin sulfate proteoglycan, an inhibitor of neural regeneration.
Shen Y, et al.
Science, 326(5952) (2009)
Charlotte Rolny et al.
The Journal of biological chemistry, 277(22), 19315-19321 (2002-03-26)
Platelet-derived growth factor (PDGF) induces mitogenic and migratory responses in a wide variety of cells, by activating specific receptor tyrosine kinases denoted the PDGF alpha- and beta-receptors. Different PDGF isoforms bind in a distinct manner to glycosaminoglycans, particularly heparan sulfate.
Effect of the counterion behavior on the frictional?compressive properties of chondroitin sulfate solutions
Polymer, 50(7), 1805-1813 (2009)
R Probstmeier et al.
The Journal of cell biology, 116(4), 1063-1070 (1992-02-01)
To gain insights into the functional role of the molecular association between neural adhesion molecules and extracellular matrix constituents, soluble forms of the myelin-associated glycoprotein (MAG) and the neural cell adhesion molecule (N-CAM), representing most of the extracellular domains of
Z Avnur et al.
Cell, 38(3), 811-822 (1984-10-01)
Chondroitin-sulfate containing proteoglycan (CSPG) of the extracellular matrix (ECM) was visualized in chick tissues and cell cultures with a monoclonal antibody, CS-56. Cultured cells of various origins contained dense punctate layers of CSPG on both the substrate and the cell

Articles

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

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