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ABN51

Sigma-Aldrich

Anti-Fox-3 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Neuronal nuclei antigen, NeuN antigen
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RBFOX3(146713)

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Anti-Fox-3 Antibody from rabbit, purified by affinity chromatography

ABN51

Anti-Fox-3 Antibody

Anti-NeuN Antibody from chicken, purified by affinity chromatography

ABN91

Anti-NeuN Antibody

Anti-NeuN Antibody (rabbit) from rabbit, purified by affinity chromatography

ABN78

Anti-NeuN Antibody (rabbit)

biological source

rabbit

biological source

rabbit

biological source

chicken

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100, 200

Quality Level

100

UniProt accession no.

Q8BIF2

UniProt accession no.

A6NFN3

UniProt accession no.

-

UniProt accession no.

Q8BIF2

species reactivity

mouse, human, rat

species reactivity

human, rat, mouse

species reactivity

mouse, rat

species reactivity

mouse, human, snail, rat

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. Fox-3 acts as a pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, and in the spinal cord (at protein level). However, Purkinje cells lack Fox-3 expression. It is also found in the retina in the ganglion cells and some cells in the inner nuclear layer, but is absent from the photoreceptor cells and most cells in the inner nuclear layer. In developing mouse it is expressed in the neural tube as early as day E9.5. Fox-3 expression is shown to be up-regulated by retinoic acid in p19 cells during neural differentiation. Knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. Five isoforms of Fox-3 have been described that are generated by alternative splicing.

Specificity

This antibody recognizes Fox-3 at the N-terminus.

Immunogen

Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse Fox-3.

Application

Anti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Western Blotting Analysis: 2 µg/mL from a representative lot detected Fox-3 in mouse brain nuclear extract.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.

Quality

Evaluated by Western Blot in mouse e16 brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.

Target description

~46 kDa observed. An uncharacterized band appears at ~68 kDa in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse e16 brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Fan Xing et al.
Cell reports, 18(2), 468-481 (2017-01-12)
Glioblastoma multiforme (GBM) is among the most aggressive of human cancers. Although differentiation therapy has been proposed as a potential approach to treat GBM, the mechanisms of induced differentiation remain poorly defined. Here, we established an induced differentiation model of GBM
Ann Kari Grindheim et al.
Journal of cell science, 129(2), 314-328 (2015-12-09)
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that
Amy E Byrnes et al.
Molecular therapy. Nucleic acids, 32, 773-793 (2023-06-22)
Antisense oligonucleotide (ASO) therapeutics are being investigated for a broad range of neurological diseases. While ASOs have been effective in the clinic, improving productive ASO internalization into target cells remains a key area of focus in the field. Here, we
Patricia Meyer et al.
Stem cells and development, 29(9), 574-585 (2020-01-23)
Hypoxic-ischemic brain injury is the leading cause of disability and death after successful resuscitation from cardiac arrest, and, to date, no specific treatment option is available to prevent subsequent neurofunctional impairments. The hippocampal cornu ammonis segment 1 (CA1) is one

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