Skip to Content
MilliporeSigma
All Photos(13)

Documents

ZMS377

Sigma-Aldrich

Anti-NeuN Antibody, clone A60 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sign Into View Organizational & Contract Pricing

Synonym(s):
Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN antigen, Neuronal nuclei antigen, RNA binding protein fox-1 homolog 3
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

A60, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 40.61 kDa
observed mol wt ~49-52 kDa

species reactivity

porcine, rat, mouse, ferret

species reactivity (predicted by homology)

human, salamander

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

Rattus norvegicus ... Rbfox3(287847)
human ... RBFOX3(146713)
mouse ... Rbfox3(52897)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone A60 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects NeuN in multiple species.

Immunogen

Purified cell nuclei from mouse brain.

Application

Anti-NeuN, clone A60 ZooMAb, Cat. No. ZMS377, is a recombinant Mouse monoclonal antibody that detects NeuN and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in Rat E18 cortical cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns and is also detected in the developing dorsal horns as well as in the dorsal root ganglion. It is expressed from embryonic stage to adulthood. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Initial characterization of NeuN was derived from usage of a monoclonal antibody (A60), but later identified as RBFOX3. Subsequent studies have shown that anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They show increased susceptibility to seizures and reduced anxiety-related behaviors compared with wild type littermates. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Greener ZooMAb® antibodies offer sustainable options with low-waste packaging and BSA-free formulation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service