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MAB1281

Sigma-Aldrich

Anti-Nuclei Antibody, clone 235-1

clone 235-1, Chemicon®, from mouse

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Synonym(s):
HuNu
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

235-1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

input

sample type neural stem cell(s)

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

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FCMAB306PMAB1281BMAB4383
vibrant-m

MAB1281

Anti-Nuclei Antibody, clone 235-1

vibrant-m

MAB4383

Anti-Nuclei Antibody, clone 3E1.3

clone

235-1, monoclonal

clone

235-1, monoclonal

clone

235-1, monoclonal

clone

3E1.3, monoclonal

species reactivity

human

species reactivity

-

species reactivity

human

species reactivity

human

antibody form

purified from hybridoma cell culture

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

isotype

IgG1

isotype

IgG1κ

isotype

IgG1

isotype

IgG1

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable (paraffin)

technique(s)

flow cytometry: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

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General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.

Specificity

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.

Application

Detect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.
Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

Identifies an 82 and 70 kD protein by immunoprecipitation.

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells

Physical form

Format: Purified
Hybridoma supernatant. Liquid in PBS. Contains no preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
All human cell types

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yuelin Zhang et al.
Cell transplantation, 21(10), 2225-2239 (2012-07-11)
Although transplantation of adult bone marrow mesenchymal stem cells (BM-MSCs) holds promise in the treatment for pulmonary arterial hypertension (PAH), the poor survival and differentiation potential of adult BM-MSCs have limited their therapeutic efficiency. Here, we compared the therapeutic efficacy
Jun Lei et al.
American journal of obstetrics and gynecology, 212(5), 639-639 (2015-01-04)
Using a mouse model of intrauterine inflammation, we have demonstrated that exposure to inflammation induces preterm birth and perinatal brain injury. Mesenchymal stem cells (MSCs) have been shown to exhibit immunomodulatory effects in many inflammatory conditions. We hypothesized that treatment
E Josue Ruiz et al.
Nature communications, 5, 3175-3175 (2014-01-17)
The signals that regulate stem cell self-renewal and differentiation in the lung remain elusive. Lung stem cells undergo self-renewal or lineage commitment to replenish tissue, depending on cross-talk with their environment. This environment, also known as the niche, includes mesenchymal
Human stem cell delivery for treatment of large segmental bone defects.
Dupont, KM; Sharma, K; Stevens, HY; Boerckel, JD; Garcia, AJ; Guldberg, RE
Proceedings of the National Academy of Sciences of the USA null
Ji Hyun Kim et al.
Scientific reports, 6, 38754-38754 (2016-12-08)
Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a preclinical rodent tissue defect; however

Articles

Leverage the inherent immune ability of an animal to generate antibodies that bind to specific molecules.

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Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

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Protocols

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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