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ABN78A4

Sigma-Aldrich

Anti-NeuN (rabbit) Antibody, Alexa Fluor 488 Conjugate

from rabbit, ALEXA FLUOR 488

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Synonym(s):
NEUronal Nuclei, Neuronal Nuclei
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

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This Item
ABN78C3MAB377C3ABN91
vibrant-m

ABN91

Anti-NeuN Antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100, 200

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

chicken

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Specificity

The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human.
This antibody recognizes NeuN at the N-terminus.

Immunogen

Epitope: N-terminus
GST-tagged recombinant protein corresponding to mouse NeuN.

application

Detect NeuN (rabbit) using this Anti-NeuN (rabbit) Antibody, Alexa Fluor 488 Conjugate validated for use in IC, IH.
Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected NeuN in rat E18 primary cortex cells.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue.

Target description

The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa An uncharacterized band appears at ~62 kDa in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Adult mouse brain tissue

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Estrogen receptor alpha deficiency protects against development of cognitive impairment in murine lupus.
Cunningham, MA; Wirth, JR; Freeman, LR; Boger, HA; Granholm, AC; Gilkeson, GS
Journal of Neuroinflammation null
Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress.
Garate, I; Garcia-Bueno, B; Madrigal, JL; Caso, JR; Alou, L; Gomez-Lus, ML; Leza, JC
Journal of Neuroinflammation null
Mingfei Zhao et al.
Molecular medicine reports, 13(1), 13-20 (2015-11-18)
Traumatic brain injury (TBI) leads to permanent neurological impairment, and methylene blue (MB) exerts central nervous system neuroprotective effects. However, only one previous study has investigated the effectiveness of MB in a controlled cortical impact injury model of TBI. In
Amanda C Mitchell et al.
Nature communications, 7, 12743-12743 (2016-09-07)
Neuronal epigenomes, including chromosomal loopings moving distal cis-regulatory elements into proximity of target genes, could serve as molecular proxy linking present-day-behaviour to past exposures. However, longitudinal assessment of chromatin state is challenging, because conventional chromosome conformation capture assays essentially provide
Alex Bersellini Farinotti et al.
The Journal of experimental medicine, 216(8), 1904-1924 (2019-06-15)
Rheumatoid arthritis-associated joint pain is frequently observed independent of disease activity, suggesting unidentified pain mechanisms. We demonstrate that antibodies binding to cartilage, specific for collagen type II (CII) or cartilage oligomeric matrix protein (COMP), elicit mechanical hypersensitivity in mice, uncoupled

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