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NuCLEAR Extraction Kit

For mammalian tissue or cultured cells

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Quality Level


 kit sufficient for 10 extractions (1 ml packed cell volume)
 kit sufficient for 100 extractions (100 μl packed cell volume)

shipped in

dry ice

storage temp.


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NuCLEAR Extraction Kit



Plant Total Protein Extraction Kit

shipped in

dry ice

shipped in


shipped in

wet ice

shipped in


storage temp.


storage temp.


storage temp.


storage temp.


Quality Level


Quality Level


Quality Level


Quality Level


General description

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.


NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

Other Notes

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.


Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Legal Information

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.

  • 3× Dilution and Equilibration Buffer 90 mL

  • P8340Protease Inhibitor Cocktail 1 mLSDS





Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class

8A - Combustible, corrosive hazardous materials




188.6 °F - closed cup


87 °C - closed cup

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F Guidez et al.
Molecular and cellular biology, 18(7), 3851-3861 (1998-06-25)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) independently stimulate the proliferation and differentiation of macrophages from bone marrow progenitor cells. Although the GM-CSF and M-CSF receptors are unrelated, both couple to Ras-dependent signal transduction pathways, suggesting that these
Darryl L Hadsell et al.
Mammalian genome : official journal of the International Mammalian Genome Society, 29(9-10), 632-655 (2018-08-04)
The breast-feeding neonate depends on mother's milk for both macronutrients and micronutrients including minerals. The goals of the present study were to document the effects of genetic background in mice on milk concentrations of select minerals and to use genome-wide
Paul R Marshall et al.
Nature neuroscience, 23(6), 718-729 (2020-05-06)
DNA forms conformational states beyond the right-handed double helix; however, the functional relevance of these noncanonical structures in the brain remains unknown. Here we show that, in the prefrontal cortex of mice, the formation of one such structure, Z-DNA, is
Céline Pinheiro et al.
Journal of translational medicine, 12, 118-118 (2014-06-03)
Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic
Yu Zheng et al.
Human reproduction (Oxford, England), 27(5), 1300-1313 (2012-03-10)
Mounting evidence shows that nuclear factor-κB (NF-κB) plays an important role in endometriosis. We therefore evaluated the therapeutic potential of andrographolide, an NF-κB inhibitor. Primary cell cultures were performed using ectopic endometrial tissue specimens and their homologous eutopic endometrial specimens


The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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