MIT1000
Mitochondria/Cytosol Fractionation Kit
This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.
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$40.81
List Price$66.90Save 39%Estimated to ship onApril 14, 2025
Select a Size
About This Item
$40.81
List Price$66.90Save 39%Estimated to ship onApril 14, 2025
Recommended Products
Quality Level
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
application(s)
sample preparation
shipped in
dry ice
1 of 4
This Item | G8142 | 14420C | I7267 |
---|---|---|---|
sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
form liquid | form liquid | form liquid | form liquid |
components NaHCO3: 0.25 g/L | components NaHCO3: 0.35 g/L | components HEPES: no | components - |
shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
Millipore’s Mitochondria/Cytosol Fractionation Kit provides reagents for quick and efficient isolation of intact mitochondria from cultured cells. This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction). Such separation is useful for studying apoptosis and signaling pathways between the two fractions, by Western blotting or ELISA.
The kit contains sufficient materials for the analysis of 50 samples.
Application
Apoptosis & Cancer
Components
Mitochondrial Lysis Buffer (CS204257): 5 mL
Protease inhibitor cocktail (CS204253): 1 mL
Anti-Bcl2 (05-729-25UG): 25 μg
Anti-GAPDH (CS204254): 25 μg
Storage and Stability
Legal Information
Disclaimer
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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