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MITOISO2

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

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Synonym(s):
Mitochondrial Extraction Kit, Mitochondrial Purification Kit
NACRES:
NA.32

Quality Level

usage

sufficient for 50 applications (2-5 x 107 cells)

technique(s)

ELISA: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

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This Item
MITOISO1CS0760MIT1000
Mitochondria Isolation Kit sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

MITOISO2

Mitochondria Isolation Kit

Mitochondria Isolation Kit sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

MITOISO1

Mitochondria Isolation Kit

Isolated Mitochondria Staining Kit 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

CS0760

Isolated Mitochondria Staining Kit

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells
  • blood samples of tuberculosis patients
  • human neuroblastoma cells and glioma cells
  • myotubes
  • HepG2 cells

Features and Benefits

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

Other Notes

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS

  • C0356Protein Extraction Reagent Type 4 1 bottleSDS

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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25G
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