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MAK160

Sigma-Aldrich

Mitochondrial Membrane Potential Kit

sufficient for 100 fluorometric tests (flow cytometry)

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Synonym(s):
JC-10 Assay
NACRES:
NA.25

usage

sufficient for 100 fluorometric tests (flow cytometry)

detection method

fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

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MAK147APT142MTP001
vibrant-m

MTP001

MITOCHONDRIAL PROTEIN IP KIT

usage

sufficient for 100 fluorometric tests (flow cytometry)

usage

sufficient for 500 fluorometric tests (orange fluorescence, microplate readers)

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

relevant disease(s)

cancer

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

-

General description

Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

Application

Mitochondrial Membrane Potential Kit has been used to detect mitochondrial membrane potential (MMP) of PC-12 cells.

Suitability

This kit is suitable for the flow cytometric detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators.

Principle

This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Intramembrane protease PARL defines a negative regulator of PINK1-and PARK2/Parkin-dependent mitophagy.
Meissner C, et al.
Autophagy, 11(9), 1484-1498 (2015)
Radix Ophiopogonis polysaccharide extracts alleviate MPP+-induced PC-12 cell injury through inhibition of Notch signaling pathway.
Liu R and Li X
International Journal of Clinical and Experimental Pathology, 11(1), 99-109 (2018)
Jianbiao Zhou et al.
Haematologica, 105(9), 2286-2297 (2020-10-16)
Differentiation therapies achieve remarkable success in acute promyelocytic leukemia, a subtype of acute myeloid leukemia. However, excluding acute promyelocytic leukemia, clinical benefits of differentiation therapies are negligible in acute myeloid leukemia except for mutant isocitrate dehydrogenase 1/2. Dihydroorotate dehydrogenase catalyses
Chaolu Chen et al.
Free radical biology & medicine, 136, 22-34 (2019-03-31)
Endometriosis is associated with inflammatory reaction, and reactive oxidative species (ROS) are highly pro-inflammatory factors. Mitochondria are responsible for the production of ROS and energy. However, little is known about how mitochondria regulate ROS generation and energy metabolism in endometriosis.
Jérôme Cléach et al.
Journal of microbiology and biotechnology, 28(11), 1782-1790 (2018-12-20)
Assessment of microorganism viability is useful in many industrial fields. A large number of methods associated with the use of fluorescent probes have been developed, including fluorimetry, fluorescence microscopy, and cytometry. In this study, a microvolume spectrofluorometer was used to

Articles

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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