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MAK144

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (orange)

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EC Number:
NACRES:
NA.25

usage

sufficient for 200 fluorometric tests (orange)

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

cancer; neurological disorders

storage temp.

−20°C

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MAK142MAK145MAK143
vibrant-m

MAK144

Fluorometric Intracellular Ros Kit

vibrant-m

MAK142

Fluorometric Intracellular Ros Kit

vibrant-m

MAK145

Fluorometric Intracellular Ros Kit

vibrant-m

MAK143

Fluorometric Intracellular Ros Kit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

sufficient for 200 fluorometric tests (orange)

usage

sufficient for 200 fluorometric tests (Deep red)

usage

sufficient for 200 fluorometric tests (red)

usage

sufficient for 200 fluorometric tests (green)

application(s)

cosmetics
food and beverages

application(s)

cosmetics
food and beverages

application(s)

-

application(s)

-

relevant disease(s)

cancer; neurological disorders

relevant disease(s)

neurological disorders; cancer

relevant disease(s)

neurological disorders; cancer

relevant disease(s)

cancer; neurological disorders

General description

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.

Application

Fluorometric intracellular reactive oxygen species (ROS) Kit has been used to evaluate the mitochondrial damage by mechanical ventilation (MV). It is also used to measure the production of intracellular ROS.

Suitability

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.

Principle

The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 540/λem = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Oxidized low density lipoprotein (ox-LDL) binding to ox-LDL receptor-1 in endothelial cells induces the activation of NF-KB through an increased production of intracellular reactive oxygen species.
Cominacini L, et al.
The Journal of Biological Chemistry, 275(17), 12633-12638 (2000)
Udayakumar Karunakaran et al.
Redox biology, 45, 102029-102029 (2021-06-10)
Prolonged hyperglycemia plays a major role in the progression of β-cell loss in diabetes mellitus. Here we report an insulin sensitizer thiazolidinedione Pioglitazone selectively preserves the beta cells against high glucose-induced dysfunction by activation of AMPK and Glutaminase 1 (GLS1)
High tidal volume induces mitochondria damage and releases mitochondrial DNA to aggravate the ventilator-induced lung injury.
Lin J, et al.
Frontiers in Immunology, 9, 1477-1477 (2018)
Feng Gao et al.
Journal of cellular biochemistry, 121(11), 4450-4457 (2020-03-13)
The mechanisms driving the pathologic progression of osteoarthritis (OA) have not yet to be fully elucidated. Excessive and irreversible breakdown of the extracellular matrix is the main hallmark of OA. Inhibitors of DPP-4 have been widely used for over a
Neurodegenerative diseases and oxidative stress.
Barnham K J, et al.
Nature Reviews. Drug Discovery, 3(3), 205-205 (2004)

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