MAK145
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (red)
Synonym(s):
Intracellular ROS Fluorescence Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.25
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 200 fluorometric tests (red)
detection method
fluorometric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
Related Categories
General description
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense.[1] Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA.[1] ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[2]
Application
Suitability
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.
Principle
The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 520/λem = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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