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MAK159

Sigma-Aldrich

Mitochondrial Membrane Potential Kit

sufficient for 500 fluorometric tests (microplate readers)

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Synonym(s):
JC-10 Assay, JC-10 Mitochondrial Membrane Potential Assay
NACRES:
NA.25

usage

sufficient for 500 fluorometric tests (microplate readers)

detection method

fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

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This Item
MAK147CS0390APT242
vibrant-m

CS0390

Mitochondria Staining Kit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

usage

sufficient for 500 fluorometric tests (microplate readers)

usage

sufficient for 500 fluorometric tests (orange fluorescence, microplate readers)

usage

 kit sufficient for 200 tests (of 1 mL cell suspensions),  kit sufficient for 40 tests (of 5 mL cell suspensions)

usage

-

relevant disease(s)

cancer

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

-

General description

Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

Application

Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.

Suitability

This kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.

Principle

This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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