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MAK166

Sigma-Aldrich

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (infrared fluorescence)

Synonym(s):

Hydrogen Peroxide Detection Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 500 fluorometric tests (infrared fluorescence)

detection method

fluorometric

relevant disease(s)

orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders

storage temp.

−20°C

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Fluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H2O2).

Features and Benefits

This assay is compatible with high-throughput handling systems.

Suitability

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principle

Fluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (λex = 640/λem = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Formation of an angular aromatic polyketide from a linear anthrene precursor via oxidative rearrangement.
Gao G, et al.
Cell Chemical Biology, 24(7), 881-891 (2017)
The mechanism of iron-compensation for manganese deficiency of Streptococcus pneumoniae.
Cao K, et al.
Journal of Proteomics, 184, 62-70 (2018)
Guixi Gao et al.
Cell chemical biology, 24(7), 881-891 (2017-07-18)
Bacterial aromatic polyketides are a group of natural products synthesized by polyketide synthases (PKSs) that show diverse structures and biological activities. They are structurally subclassified into linear, angular, and discoid aromatic polyketides, the formation of which is commonly determined by
Fritz Schweikart et al.
Analytical chemistry, 91(3), 1748-1751 (2019-01-24)
HPLC coupled to both UV and MS is an established setup for purity assessments in many areas. With evolving technology, instrument sensitivity increases, calling for lower sample concentration, while light flux in a commercial UV detector cell is considerably higher
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Journal of inflammation research, 14, 4053-4067 (2021-08-31)
Milk depression is the major driver of economic loss due to mastitis in dairy animals. The aim of this study was to identify potential mediators of milk depression by investigating the local and systemic changes in gene expression or cytokine

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