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11467140001

Roche

DNA, MB-grade

from fish sperm

Synonym(s):

DNA

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About This Item

UNSPSC Code:
41106310

biological source

fish (sperm)

Quality Level

grade

Molecular Biology

form

solution (ready-to-use)

mol wt

40-1,000 kDa

packaging

pkg of 500 mg

manufacturer/tradename

Roche

concentration

100 μg/mL

color

light yellowopalescent

solubility

water: miscible

storage temp.

−20°C

General description

DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.

Application

The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.

Sequence

Chain Length 120 to 3,000 nucleotides

Physical form

Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0

Preparation Note

Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available


Certificates of Analysis (COA)

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Yu Sasano et al.
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In humans and other holozoan organisms, the ribosomal protein eS30 is synthesized as a fusion protein with the ubiquitin-like protein FUBI. However, FUBI is not part of the mature 40S ribosomal subunit and cleaved off by an as-of-yet unidentified protease.
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The MAPKs ERK, JNK, and p38 control diverse aspects of the immune response, including regulation of cytotoxin biology in NK cells and CTL. The chemokine CCL5 is coreleased with the cytotoxins, perforin, the granzymes, and granulysin, during the lethal hit

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