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MAC4L

Sigma-Aldrich

MetaPolyzyme

lyophilized powder

Synonym(s):

Multilytic Enzyme Mix

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

technique(s)

DNA extraction: suitable

suitability

suitable for microbiology

application(s)

microbiology

shipped in

wet ice

storage temp.

−20°C

General description

Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.

Application

MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG). Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.

Biochem/physiol Actions

Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.

Components

The enzymes in MetaPolyzme are:
  • Achromopeptidase
  • Chitinase
  • Lyticase
  • Lysostaphin
  • Lysozyme
  • Mutanolysin

Unit Definition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maria A Sierra et al.
Environmental microbiome, 17(1), 60-60 (2022-12-22)
Lake Hillier is a hypersaline lake known for its distinctive bright pink color. The cause of this phenomenon in other hypersaline sites has been attributed to halophiles, Dunaliella, and Salinibacter, however, a systematic analysis of the microbial communities, their functional
Erratum to: The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium Inaugural Meeting Report.
Microbiome, 4(1), 45-45 (2016-08-20)
Scott Tighe et al.
Journal of biomolecular techniques : JBT, 28(1), 31-39 (2017-03-25)
The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques.
Steve Hamner et al.
International journal of environmental research and public health, 16(7), 1097-1097 (2019-04-03)
The Little Bighorn River is the primary source of water for water treatment plants serving the local Crow Agency population, and has special significance in the spiritual and ceremonial life of the Crow tribe. Unfortunately, the watershed suffers from impaired
Cartography of Opportunistic Pathogens and Antibiotic Resistance Genes in a Tertiary Hospital Environment.
Chng KR et al.
Nature Medicine, 26(6), 941-951 (2020)

Articles

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

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