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P8806

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

tablet, 3 mg substrate per tablet

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5 X 50 MG
$403.00

$403.00

Estimated to ship onMay 12, 2025

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5 X 50 MG
$403.00

About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein:
3912045
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898981
NACRES:
NA.83

$403.00

Estimated to ship onMay 12, 2025

To order products, please contact your local dealer.

Quality Level

200

form

tablet

mp

258 °C (dec.) (lit.)

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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This Item
C9995C0871C7117
Anti-Cullin-4A antibody produced in rabbit ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

C0371

Anti-Cullin-4A antibody produced in rabbit

Anti-Cullin-4B antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

C9995

Anti-Cullin-4B antibody produced in rabbit

Anti-Cullin 3 antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

C0871

Anti-Cullin 3 antibody produced in rabbit

Anti-Cullin-1 antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

C7117

Anti-Cullin-1 antibody produced in rabbit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

indirect immunofluorescence: 10-20 μg/mL using HeLa cells, western blot: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A

technique(s)

western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B.

technique(s)

immunoprecipitation (IP): 5-10 μg using lysate of rat brain, indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells, western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3

technique(s)

western blot: 0.5-1 μg/mL using whole cell extract of human colon carcinoma HCT-116 cell line and whole cell extract of mouse fibroblasts NIH3T3 cell line

UniProt accession no.

Q13619

UniProt accession no.

Q13620

UniProt accession no.

Q13618

UniProt accession no.

Q13616

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Signal Word

Warning

Hazard Statements

H317,H341,H351,H411

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000312484
0000258657
SLBN7267V
SLBR6541V
SLBJ5969V

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