70152

Plate Count Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Casein-peptone Dextrose Yeast Agar, Standard Methods Agar, Tryptone Glucose Yeast Extract Agar
• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• Agency: according to GB 4789.2-2016; according to ISO 17410:2019; according to ISO 4833-1:2013; according to ISO 4833-2:2013
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 9.0 g/L ; dextrose, 1.0 g/L ; tryptone, 5.0 g/L ; yeast extract, 2.5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• packaging: pkg of -2.5 kg; pkg of -500 g
• storage condition: (Dry place, tightly sealed containers)
• technique(s): microbiological culture: suitable
• final pH: 7.0±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring; microbiology
• storage temp.: 2-25°C
• suitability: nonselective for Bacillus spp.; nonselective for Escherichia coli; nonselective for Lactobacillus spp.; nonselective for Lactococcus spp.; nonselective for Listeria spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Plate Count Agar is the recommended medium forthe isolation of microorganisms in milk and other dairy products. The enzymaticdigest of casein (tryptone) with a high level of free amino acids is a nitrogensource. Yeast extract primarily supplies the B-complex vitamins. Glucose is anenergy source for the growth of bacteria, while agar is the solidifying agent.Plate Count Agar is also suitable for determining bacterial count from sterilerooms.

Application

Plate Count Agar is a non-selective medium for the plate count of microorganisms in water, food, milk, dairy products, beverages, animal feed, and other materials.

Preparation Note

Suspend 17.5 g in 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Comparison of β-glucuronidase and indole-based direct plating methods for enumeration of unstressed E. coli

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves