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U6253

Sigma-Aldrich

Ubiquitin from bovine erythrocytes

BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

ATP-dependent proteolytic factor, Ub

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

bovine erythrocytes

Quality Level

product line

BioUltra

Assay

≥98% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

storage condition

(Tightly closed. Dry)

technique(s)

western blot: suitable

impurities

salt, essentially free

solubility

water: 1 mg/mL, clear, colorless

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... LOC(101902760)

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General description

Research area: Cancer

Ubiquitin is a highly conserved regulatory protein. It is found in all eukaryotic cells and is virtually identical across all forms of life including yeast, humans, and plants. ubiquitin structure contains seven Lys residues and an N-terminus, all of which are target sites for ubiquitination.
This product is from bovine red blood cells. It is not a recombinant product. The production process is considered proprietary, however it includes heat treatment as well as purification by chromatography and dialysis.

Application

Ubiquitin from bovine erythrocytes has been used to study the role of exogenous ubiquitin in chronic β-adrenergic receptor (β-AR)-stimulated myocardial remodeling. It has also been used to test the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) ubiquitylation.

Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.

Biochem/physiol Actions

Ubiquitination is a post-translational modification process where ubiquitin-protein is attached to a substrate protein. Ubiquitination plays a vital role in the regulation of cellular signaling in various biological processes such as apoptosis, protein processing, immune response, and DNA repair. Ubiquitination mediates protein degradation via the ubiquitin-proteasome pathway. Ubiquitination is implicated in various cellular signaling pathways. Polyubiquitination modulates the signal activation of NF-κ-B inhibitor alpha (IkB-α) in the inflammatory signaling pathway. Elevated levels of ubiquitin have been observed in various diseases such as parasitic and allergic diseases, alcoholic liver disease, type 2 diabetes, β2-microglobulin amyloidosis, and chronic hemodialysis.

Preparation Note

Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A L Schwartz et al.
Annual review of medicine, 50, 57-74 (1999-03-12)
The ubiquitin-proteasome pathway plays a pivotal role in the degradation of short-lived and regulatory proteins important in a variety of basic cellular processes, including regulation of the cell cycle, modulation of cell surface receptors and ion channels, and antigen presentation.
Biochemistry, Ubiquitination
Guo HJ, et al.
StatPearls [Internet] (2022)
Takeshi Miura et al.
FEMS microbiology letters, 239(1), 171-179 (2004-09-29)
When Saccharomyces cerevisiae cells are exposed to high hydrostatic pressure, tryptophan permease Tat2 is degraded in a manner dependent on Rsp5 ubiquitin ligase. Consequently, cell growth is arrested in tryptophan auxotrophic strains. Here we show that of 17 ubiquitin-specific protease
The ubiquitin system for protein degradation.
A Hershko et al.
Annual review of biochemistry, 61, 761-807 (1992-01-01)
Errol W Robinson et al.
Analytical chemistry, 80(19), 7508-7515 (2008-08-30)
Field asymmetric waveform ion mobility spectrometry (FAIMS) has emerged as an analytical tool of broad utility, especially in conjunction with mass spectrometry. Of particular promise is the use of FAIMS and 2-D ion mobility methods that combine FAIMS with conventional

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